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plasmid construct apex2 plasmid addgene  (Addgene inc)


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    Addgene inc plasmid construct apex2 plasmid addgene
    Plasmid Construct Apex2 Plasmid Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+construct+apex2+plasmid+addgene/pGEX-2TK-WW-EF1%2BEF2+(Plasmid+%2318080)/pm31989825__pr9b00609_si_001-71-134-138
    Average 90 stars, based on 1 article reviews
    plasmid construct apex2 plasmid addgene - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Lysis:

    Article Title: Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.
    Article Snippet: Baker 409704 3mM calcium chloride Merck 1.02382.1000 2mM magnesium acetate Merck 1.05819.0250 0.1mM EDTA Invitrogen 15575-038 10mM DTT Merck 1.11474.0005 0.5mM PMSF Sigma P7626 Nuclei lysis buffer 50mM HEPES Sigma H4034 3mM magnesium chloride Sigma M8266 300mM sodium chloride Merck Millipore 1.06404.5000 1mM DTT Merck 1.11474.0005 0.1mM PMSF Merck Millipore P7626 0.1mg/ml DNase I Cyrusbioscience 101.9003.98.9 Reagents and resource Brand Catalog Cell culture Dulbecco’s modified Eagle’s medium Gibco 12100-046 10% fetal bovine serum Gibco 10437-028 sodium bicarbonate Sigma S5761 polyethyleneimine (linear, MW25000) Polyscience 23966-1 Opti-MEM Gibco 31985-070 polybrene Merck Millipore TR-1003-G puromycin Invivogen ant-pr-1 Versene buffer 0.5 mM EDTA Invitrogen 15575-038 Cell culture PBS Corning 46-013-CM Reagents and resource Brand Catalog S-14 RNA extraction and reverse-transcription PCR RNeasy mini kit Qiagen 74106 70% ethanol Merck Millipore 1.00983.2500 SuperScript® III First-strand synthesis system Invitrogen 18080-051 Plasmid Construct APEX2 plasmid Addgene #49386 BASU plasmid Addgene #107250 Phusion polymerase New England BioLabs M0530 1% agarose Amresco 0710 GenepHlowTM Gel/PCR kit Geneaid DFH300 Stbl NEB C3040 mini-prep extraction kit Geneaid PDH301 Reagents and resource Brand Catalog PL/XL-MS low-binding eppendorf Eppendorf 0030 120-086 streptavidin magnetic beads Promega Z5481 disuccinimidyl suberate Cayman 13008 2M urea Amresco 0378 ammonium bicarbonate Sigma 9830 tris(2-carboxyethyl)phosphine Sigma C4706 iodoacetamide Sigma I1149 trypsin Promega V511A 10% Trifluoroacetic Acid Merck 1.08262.1000 Reagents and resource Brand Catalog Antibodies Anti-myc-AF647 Cell signalling 2233S Anti-streptavidin-HRP Rockland S000-03 Anti-streptavidin-FITC Rockland S000-02 Anti-HA-AF488 Biolegend 901509 Anti-HA-AF647 Biolegend 682404 S-15 Table S2.

    Cell Culture:

    Article Title: Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.
    Article Snippet: Baker 409704 3mM calcium chloride Merck 1.02382.1000 2mM magnesium acetate Merck 1.05819.0250 0.1mM EDTA Invitrogen 15575-038 10mM DTT Merck 1.11474.0005 0.5mM PMSF Sigma P7626 Nuclei lysis buffer 50mM HEPES Sigma H4034 3mM magnesium chloride Sigma M8266 300mM sodium chloride Merck Millipore 1.06404.5000 1mM DTT Merck 1.11474.0005 0.1mM PMSF Merck Millipore P7626 0.1mg/ml DNase I Cyrusbioscience 101.9003.98.9 Reagents and resource Brand Catalog Cell culture Dulbecco’s modified Eagle’s medium Gibco 12100-046 10% fetal bovine serum Gibco 10437-028 sodium bicarbonate Sigma S5761 polyethyleneimine (linear, MW25000) Polyscience 23966-1 Opti-MEM Gibco 31985-070 polybrene Merck Millipore TR-1003-G puromycin Invivogen ant-pr-1 Versene buffer 0.5 mM EDTA Invitrogen 15575-038 Cell culture PBS Corning 46-013-CM Reagents and resource Brand Catalog S-14 RNA extraction and reverse-transcription PCR RNeasy mini kit Qiagen 74106 70% ethanol Merck Millipore 1.00983.2500 SuperScript® III First-strand synthesis system Invitrogen 18080-051 Plasmid Construct APEX2 plasmid Addgene #49386 BASU plasmid Addgene #107250 Phusion polymerase New England BioLabs M0530 1% agarose Amresco 0710 GenepHlowTM Gel/PCR kit Geneaid DFH300 Stbl NEB C3040 mini-prep extraction kit Geneaid PDH301 Reagents and resource Brand Catalog PL/XL-MS low-binding eppendorf Eppendorf 0030 120-086 streptavidin magnetic beads Promega Z5481 disuccinimidyl suberate Cayman 13008 2M urea Amresco 0378 ammonium bicarbonate Sigma 9830 tris(2-carboxyethyl)phosphine Sigma C4706 iodoacetamide Sigma I1149 trypsin Promega V511A 10% Trifluoroacetic Acid Merck 1.08262.1000 Reagents and resource Brand Catalog Antibodies Anti-myc-AF647 Cell signalling 2233S Anti-streptavidin-HRP Rockland S000-03 Anti-streptavidin-FITC Rockland S000-02 Anti-HA-AF488 Biolegend 901509 Anti-HA-AF647 Biolegend 682404 S-15 Table S2.

    Modification:

    Article Title: Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.
    Article Snippet: Baker 409704 3mM calcium chloride Merck 1.02382.1000 2mM magnesium acetate Merck 1.05819.0250 0.1mM EDTA Invitrogen 15575-038 10mM DTT Merck 1.11474.0005 0.5mM PMSF Sigma P7626 Nuclei lysis buffer 50mM HEPES Sigma H4034 3mM magnesium chloride Sigma M8266 300mM sodium chloride Merck Millipore 1.06404.5000 1mM DTT Merck 1.11474.0005 0.1mM PMSF Merck Millipore P7626 0.1mg/ml DNase I Cyrusbioscience 101.9003.98.9 Reagents and resource Brand Catalog Cell culture Dulbecco’s modified Eagle’s medium Gibco 12100-046 10% fetal bovine serum Gibco 10437-028 sodium bicarbonate Sigma S5761 polyethyleneimine (linear, MW25000) Polyscience 23966-1 Opti-MEM Gibco 31985-070 polybrene Merck Millipore TR-1003-G puromycin Invivogen ant-pr-1 Versene buffer 0.5 mM EDTA Invitrogen 15575-038 Cell culture PBS Corning 46-013-CM Reagents and resource Brand Catalog S-14 RNA extraction and reverse-transcription PCR RNeasy mini kit Qiagen 74106 70% ethanol Merck Millipore 1.00983.2500 SuperScript® III First-strand synthesis system Invitrogen 18080-051 Plasmid Construct APEX2 plasmid Addgene #49386 BASU plasmid Addgene #107250 Phusion polymerase New England BioLabs M0530 1% agarose Amresco 0710 GenepHlowTM Gel/PCR kit Geneaid DFH300 Stbl NEB C3040 mini-prep extraction kit Geneaid PDH301 Reagents and resource Brand Catalog PL/XL-MS low-binding eppendorf Eppendorf 0030 120-086 streptavidin magnetic beads Promega Z5481 disuccinimidyl suberate Cayman 13008 2M urea Amresco 0378 ammonium bicarbonate Sigma 9830 tris(2-carboxyethyl)phosphine Sigma C4706 iodoacetamide Sigma I1149 trypsin Promega V511A 10% Trifluoroacetic Acid Merck 1.08262.1000 Reagents and resource Brand Catalog Antibodies Anti-myc-AF647 Cell signalling 2233S Anti-streptavidin-HRP Rockland S000-03 Anti-streptavidin-FITC Rockland S000-02 Anti-HA-AF488 Biolegend 901509 Anti-HA-AF647 Biolegend 682404 S-15 Table S2.

    RNA Extraction:

    Article Title: Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.
    Article Snippet: Baker 409704 3mM calcium chloride Merck 1.02382.1000 2mM magnesium acetate Merck 1.05819.0250 0.1mM EDTA Invitrogen 15575-038 10mM DTT Merck 1.11474.0005 0.5mM PMSF Sigma P7626 Nuclei lysis buffer 50mM HEPES Sigma H4034 3mM magnesium chloride Sigma M8266 300mM sodium chloride Merck Millipore 1.06404.5000 1mM DTT Merck 1.11474.0005 0.1mM PMSF Merck Millipore P7626 0.1mg/ml DNase I Cyrusbioscience 101.9003.98.9 Reagents and resource Brand Catalog Cell culture Dulbecco’s modified Eagle’s medium Gibco 12100-046 10% fetal bovine serum Gibco 10437-028 sodium bicarbonate Sigma S5761 polyethyleneimine (linear, MW25000) Polyscience 23966-1 Opti-MEM Gibco 31985-070 polybrene Merck Millipore TR-1003-G puromycin Invivogen ant-pr-1 Versene buffer 0.5 mM EDTA Invitrogen 15575-038 Cell culture PBS Corning 46-013-CM Reagents and resource Brand Catalog S-14 RNA extraction and reverse-transcription PCR RNeasy mini kit Qiagen 74106 70% ethanol Merck Millipore 1.00983.2500 SuperScript® III First-strand synthesis system Invitrogen 18080-051 Plasmid Construct APEX2 plasmid Addgene #49386 BASU plasmid Addgene #107250 Phusion polymerase New England BioLabs M0530 1% agarose Amresco 0710 GenepHlowTM Gel/PCR kit Geneaid DFH300 Stbl NEB C3040 mini-prep extraction kit Geneaid PDH301 Reagents and resource Brand Catalog PL/XL-MS low-binding eppendorf Eppendorf 0030 120-086 streptavidin magnetic beads Promega Z5481 disuccinimidyl suberate Cayman 13008 2M urea Amresco 0378 ammonium bicarbonate Sigma 9830 tris(2-carboxyethyl)phosphine Sigma C4706 iodoacetamide Sigma I1149 trypsin Promega V511A 10% Trifluoroacetic Acid Merck 1.08262.1000 Reagents and resource Brand Catalog Antibodies Anti-myc-AF647 Cell signalling 2233S Anti-streptavidin-HRP Rockland S000-03 Anti-streptavidin-FITC Rockland S000-02 Anti-HA-AF488 Biolegend 901509 Anti-HA-AF647 Biolegend 682404 S-15 Table S2.

    Reverse Transcription:

    Article Title: Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.
    Article Snippet: Baker 409704 3mM calcium chloride Merck 1.02382.1000 2mM magnesium acetate Merck 1.05819.0250 0.1mM EDTA Invitrogen 15575-038 10mM DTT Merck 1.11474.0005 0.5mM PMSF Sigma P7626 Nuclei lysis buffer 50mM HEPES Sigma H4034 3mM magnesium chloride Sigma M8266 300mM sodium chloride Merck Millipore 1.06404.5000 1mM DTT Merck 1.11474.0005 0.1mM PMSF Merck Millipore P7626 0.1mg/ml DNase I Cyrusbioscience 101.9003.98.9 Reagents and resource Brand Catalog Cell culture Dulbecco’s modified Eagle’s medium Gibco 12100-046 10% fetal bovine serum Gibco 10437-028 sodium bicarbonate Sigma S5761 polyethyleneimine (linear, MW25000) Polyscience 23966-1 Opti-MEM Gibco 31985-070 polybrene Merck Millipore TR-1003-G puromycin Invivogen ant-pr-1 Versene buffer 0.5 mM EDTA Invitrogen 15575-038 Cell culture PBS Corning 46-013-CM Reagents and resource Brand Catalog S-14 RNA extraction and reverse-transcription PCR RNeasy mini kit Qiagen 74106 70% ethanol Merck Millipore 1.00983.2500 SuperScript® III First-strand synthesis system Invitrogen 18080-051 Plasmid Construct APEX2 plasmid Addgene #49386 BASU plasmid Addgene #107250 Phusion polymerase New England BioLabs M0530 1% agarose Amresco 0710 GenepHlowTM Gel/PCR kit Geneaid DFH300 Stbl NEB C3040 mini-prep extraction kit Geneaid PDH301 Reagents and resource Brand Catalog PL/XL-MS low-binding eppendorf Eppendorf 0030 120-086 streptavidin magnetic beads Promega Z5481 disuccinimidyl suberate Cayman 13008 2M urea Amresco 0378 ammonium bicarbonate Sigma 9830 tris(2-carboxyethyl)phosphine Sigma C4706 iodoacetamide Sigma I1149 trypsin Promega V511A 10% Trifluoroacetic Acid Merck 1.08262.1000 Reagents and resource Brand Catalog Antibodies Anti-myc-AF647 Cell signalling 2233S Anti-streptavidin-HRP Rockland S000-03 Anti-streptavidin-FITC Rockland S000-02 Anti-HA-AF488 Biolegend 901509 Anti-HA-AF647 Biolegend 682404 S-15 Table S2.

    Polymerase Chain Reaction:

    Article Title: Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.
    Article Snippet: Baker 409704 3mM calcium chloride Merck 1.02382.1000 2mM magnesium acetate Merck 1.05819.0250 0.1mM EDTA Invitrogen 15575-038 10mM DTT Merck 1.11474.0005 0.5mM PMSF Sigma P7626 Nuclei lysis buffer 50mM HEPES Sigma H4034 3mM magnesium chloride Sigma M8266 300mM sodium chloride Merck Millipore 1.06404.5000 1mM DTT Merck 1.11474.0005 0.1mM PMSF Merck Millipore P7626 0.1mg/ml DNase I Cyrusbioscience 101.9003.98.9 Reagents and resource Brand Catalog Cell culture Dulbecco’s modified Eagle’s medium Gibco 12100-046 10% fetal bovine serum Gibco 10437-028 sodium bicarbonate Sigma S5761 polyethyleneimine (linear, MW25000) Polyscience 23966-1 Opti-MEM Gibco 31985-070 polybrene Merck Millipore TR-1003-G puromycin Invivogen ant-pr-1 Versene buffer 0.5 mM EDTA Invitrogen 15575-038 Cell culture PBS Corning 46-013-CM Reagents and resource Brand Catalog S-14 RNA extraction and reverse-transcription PCR RNeasy mini kit Qiagen 74106 70% ethanol Merck Millipore 1.00983.2500 SuperScript® III First-strand synthesis system Invitrogen 18080-051 Plasmid Construct APEX2 plasmid Addgene #49386 BASU plasmid Addgene #107250 Phusion polymerase New England BioLabs M0530 1% agarose Amresco 0710 GenepHlowTM Gel/PCR kit Geneaid DFH300 Stbl NEB C3040 mini-prep extraction kit Geneaid PDH301 Reagents and resource Brand Catalog PL/XL-MS low-binding eppendorf Eppendorf 0030 120-086 streptavidin magnetic beads Promega Z5481 disuccinimidyl suberate Cayman 13008 2M urea Amresco 0378 ammonium bicarbonate Sigma 9830 tris(2-carboxyethyl)phosphine Sigma C4706 iodoacetamide Sigma I1149 trypsin Promega V511A 10% Trifluoroacetic Acid Merck 1.08262.1000 Reagents and resource Brand Catalog Antibodies Anti-myc-AF647 Cell signalling 2233S Anti-streptavidin-HRP Rockland S000-03 Anti-streptavidin-FITC Rockland S000-02 Anti-HA-AF488 Biolegend 901509 Anti-HA-AF647 Biolegend 682404 S-15 Table S2.

    Plasmid Preparation:

    Article Title: Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.
    Article Snippet: Baker 409704 3mM calcium chloride Merck 1.02382.1000 2mM magnesium acetate Merck 1.05819.0250 0.1mM EDTA Invitrogen 15575-038 10mM DTT Merck 1.11474.0005 0.5mM PMSF Sigma P7626 Nuclei lysis buffer 50mM HEPES Sigma H4034 3mM magnesium chloride Sigma M8266 300mM sodium chloride Merck Millipore 1.06404.5000 1mM DTT Merck 1.11474.0005 0.1mM PMSF Merck Millipore P7626 0.1mg/ml DNase I Cyrusbioscience 101.9003.98.9 Reagents and resource Brand Catalog Cell culture Dulbecco’s modified Eagle’s medium Gibco 12100-046 10% fetal bovine serum Gibco 10437-028 sodium bicarbonate Sigma S5761 polyethyleneimine (linear, MW25000) Polyscience 23966-1 Opti-MEM Gibco 31985-070 polybrene Merck Millipore TR-1003-G puromycin Invivogen ant-pr-1 Versene buffer 0.5 mM EDTA Invitrogen 15575-038 Cell culture PBS Corning 46-013-CM Reagents and resource Brand Catalog S-14 RNA extraction and reverse-transcription PCR RNeasy mini kit Qiagen 74106 70% ethanol Merck Millipore 1.00983.2500 SuperScript® III First-strand synthesis system Invitrogen 18080-051 Plasmid Construct APEX2 plasmid Addgene #49386 BASU plasmid Addgene #107250 Phusion polymerase New England BioLabs M0530 1% agarose Amresco 0710 GenepHlowTM Gel/PCR kit Geneaid DFH300 Stbl NEB C3040 mini-prep extraction kit Geneaid PDH301 Reagents and resource Brand Catalog PL/XL-MS low-binding eppendorf Eppendorf 0030 120-086 streptavidin magnetic beads Promega Z5481 disuccinimidyl suberate Cayman 13008 2M urea Amresco 0378 ammonium bicarbonate Sigma 9830 tris(2-carboxyethyl)phosphine Sigma C4706 iodoacetamide Sigma I1149 trypsin Promega V511A 10% Trifluoroacetic Acid Merck 1.08262.1000 Reagents and resource Brand Catalog Antibodies Anti-myc-AF647 Cell signalling 2233S Anti-streptavidin-HRP Rockland S000-03 Anti-streptavidin-FITC Rockland S000-02 Anti-HA-AF488 Biolegend 901509 Anti-HA-AF647 Biolegend 682404 S-15 Table S2.

    Construct:

    Article Title: Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.
    Article Snippet: Baker 409704 3mM calcium chloride Merck 1.02382.1000 2mM magnesium acetate Merck 1.05819.0250 0.1mM EDTA Invitrogen 15575-038 10mM DTT Merck 1.11474.0005 0.5mM PMSF Sigma P7626 Nuclei lysis buffer 50mM HEPES Sigma H4034 3mM magnesium chloride Sigma M8266 300mM sodium chloride Merck Millipore 1.06404.5000 1mM DTT Merck 1.11474.0005 0.1mM PMSF Merck Millipore P7626 0.1mg/ml DNase I Cyrusbioscience 101.9003.98.9 Reagents and resource Brand Catalog Cell culture Dulbecco’s modified Eagle’s medium Gibco 12100-046 10% fetal bovine serum Gibco 10437-028 sodium bicarbonate Sigma S5761 polyethyleneimine (linear, MW25000) Polyscience 23966-1 Opti-MEM Gibco 31985-070 polybrene Merck Millipore TR-1003-G puromycin Invivogen ant-pr-1 Versene buffer 0.5 mM EDTA Invitrogen 15575-038 Cell culture PBS Corning 46-013-CM Reagents and resource Brand Catalog S-14 RNA extraction and reverse-transcription PCR RNeasy mini kit Qiagen 74106 70% ethanol Merck Millipore 1.00983.2500 SuperScript® III First-strand synthesis system Invitrogen 18080-051 Plasmid Construct APEX2 plasmid Addgene #49386 BASU plasmid Addgene #107250 Phusion polymerase New England BioLabs M0530 1% agarose Amresco 0710 GenepHlowTM Gel/PCR kit Geneaid DFH300 Stbl NEB C3040 mini-prep extraction kit Geneaid PDH301 Reagents and resource Brand Catalog PL/XL-MS low-binding eppendorf Eppendorf 0030 120-086 streptavidin magnetic beads Promega Z5481 disuccinimidyl suberate Cayman 13008 2M urea Amresco 0378 ammonium bicarbonate Sigma 9830 tris(2-carboxyethyl)phosphine Sigma C4706 iodoacetamide Sigma I1149 trypsin Promega V511A 10% Trifluoroacetic Acid Merck 1.08262.1000 Reagents and resource Brand Catalog Antibodies Anti-myc-AF647 Cell signalling 2233S Anti-streptavidin-HRP Rockland S000-03 Anti-streptavidin-FITC Rockland S000-02 Anti-HA-AF488 Biolegend 901509 Anti-HA-AF647 Biolegend 682404 S-15 Table S2.

    Extraction:

    Article Title: Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.
    Article Snippet: Baker 409704 3mM calcium chloride Merck 1.02382.1000 2mM magnesium acetate Merck 1.05819.0250 0.1mM EDTA Invitrogen 15575-038 10mM DTT Merck 1.11474.0005 0.5mM PMSF Sigma P7626 Nuclei lysis buffer 50mM HEPES Sigma H4034 3mM magnesium chloride Sigma M8266 300mM sodium chloride Merck Millipore 1.06404.5000 1mM DTT Merck 1.11474.0005 0.1mM PMSF Merck Millipore P7626 0.1mg/ml DNase I Cyrusbioscience 101.9003.98.9 Reagents and resource Brand Catalog Cell culture Dulbecco’s modified Eagle’s medium Gibco 12100-046 10% fetal bovine serum Gibco 10437-028 sodium bicarbonate Sigma S5761 polyethyleneimine (linear, MW25000) Polyscience 23966-1 Opti-MEM Gibco 31985-070 polybrene Merck Millipore TR-1003-G puromycin Invivogen ant-pr-1 Versene buffer 0.5 mM EDTA Invitrogen 15575-038 Cell culture PBS Corning 46-013-CM Reagents and resource Brand Catalog S-14 RNA extraction and reverse-transcription PCR RNeasy mini kit Qiagen 74106 70% ethanol Merck Millipore 1.00983.2500 SuperScript® III First-strand synthesis system Invitrogen 18080-051 Plasmid Construct APEX2 plasmid Addgene #49386 BASU plasmid Addgene #107250 Phusion polymerase New England BioLabs M0530 1% agarose Amresco 0710 GenepHlowTM Gel/PCR kit Geneaid DFH300 Stbl NEB C3040 mini-prep extraction kit Geneaid PDH301 Reagents and resource Brand Catalog PL/XL-MS low-binding eppendorf Eppendorf 0030 120-086 streptavidin magnetic beads Promega Z5481 disuccinimidyl suberate Cayman 13008 2M urea Amresco 0378 ammonium bicarbonate Sigma 9830 tris(2-carboxyethyl)phosphine Sigma C4706 iodoacetamide Sigma I1149 trypsin Promega V511A 10% Trifluoroacetic Acid Merck 1.08262.1000 Reagents and resource Brand Catalog Antibodies Anti-myc-AF647 Cell signalling 2233S Anti-streptavidin-HRP Rockland S000-03 Anti-streptavidin-FITC Rockland S000-02 Anti-HA-AF488 Biolegend 901509 Anti-HA-AF647 Biolegend 682404 S-15 Table S2.

    Magnetic Beads:

    Article Title: Combining Proximity Labeling and Cross-Linking Mass Spectrometry for Proteomic Dissection of Nuclear Envelope Interactome.
    Article Snippet: Baker 409704 3mM calcium chloride Merck 1.02382.1000 2mM magnesium acetate Merck 1.05819.0250 0.1mM EDTA Invitrogen 15575-038 10mM DTT Merck 1.11474.0005 0.5mM PMSF Sigma P7626 Nuclei lysis buffer 50mM HEPES Sigma H4034 3mM magnesium chloride Sigma M8266 300mM sodium chloride Merck Millipore 1.06404.5000 1mM DTT Merck 1.11474.0005 0.1mM PMSF Merck Millipore P7626 0.1mg/ml DNase I Cyrusbioscience 101.9003.98.9 Reagents and resource Brand Catalog Cell culture Dulbecco’s modified Eagle’s medium Gibco 12100-046 10% fetal bovine serum Gibco 10437-028 sodium bicarbonate Sigma S5761 polyethyleneimine (linear, MW25000) Polyscience 23966-1 Opti-MEM Gibco 31985-070 polybrene Merck Millipore TR-1003-G puromycin Invivogen ant-pr-1 Versene buffer 0.5 mM EDTA Invitrogen 15575-038 Cell culture PBS Corning 46-013-CM Reagents and resource Brand Catalog S-14 RNA extraction and reverse-transcription PCR RNeasy mini kit Qiagen 74106 70% ethanol Merck Millipore 1.00983.2500 SuperScript® III First-strand synthesis system Invitrogen 18080-051 Plasmid Construct APEX2 plasmid Addgene #49386 BASU plasmid Addgene #107250 Phusion polymerase New England BioLabs M0530 1% agarose Amresco 0710 GenepHlowTM Gel/PCR kit Geneaid DFH300 Stbl NEB C3040 mini-prep extraction kit Geneaid PDH301 Reagents and resource Brand Catalog PL/XL-MS low-binding eppendorf Eppendorf 0030 120-086 streptavidin magnetic beads Promega Z5481 disuccinimidyl suberate Cayman 13008 2M urea Amresco 0378 ammonium bicarbonate Sigma 9830 tris(2-carboxyethyl)phosphine Sigma C4706 iodoacetamide Sigma I1149 trypsin Promega V511A 10% Trifluoroacetic Acid Merck 1.08262.1000 Reagents and resource Brand Catalog Antibodies Anti-myc-AF647 Cell signalling 2233S Anti-streptavidin-HRP Rockland S000-03 Anti-streptavidin-FITC Rockland S000-02 Anti-HA-AF488 Biolegend 901509 Anti-HA-AF647 Biolegend 682404 S-15 Table S2.



    Similar Products

    plasmid construct apex2 plasmid addgene  (Addgene inc)
    90
    Addgene inc plasmid construct apex2 plasmid addgene
    Plasmid Construct Apex2 Plasmid Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+construct+apex2+plasmid+addgene/pGEX-2TK-WW-EF1%2BEF2+(Plasmid+%2318080)/pm31989825__pr9b00609_si_001-71-134-138
    Average 90 stars, based on 1 article reviews
    plasmid construct apex2 plasmid addgene - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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